Cryopreservation and vitrification
Cryopreservation is a method of preserving and freezing reproductive cells using special media for preservation in liquid nitrogen at -196 °C. This method can preserve reproductive cells for many (even decades).
For freezing eggs and embryos we use the modern method of vitrification, where the reproductive cells are cooled down to a temperature of -196 °C in seconds. This uses a medium containing high doses of cryoprotective substances that protect the cells from damage.
At our clinic we store reproductive cells in a unique modern cryobank. The cryobank is fully automated and ensures optimal storage conditions for samples 24 hours a day. The stored samples are protected from temperature fluctuations, lack of liquid nitrogen or careless handling, unlike the commonly used Dewar vessels. Access to the cryobank is restricted and permanently monitored against misuse.
Freezing of embryos
Vitrification of embryos is carried out using a modern technique in a closed system using an inner carrier and an outer shell. The embryos are almost immediately frozen. Compared to slow freezing, this system has increased the success rate of infertility treatment using frozen embryos almost to the level of fresh embryos.
When do embryos freeze?
Vitrification is part of IVF treatment
The high success rate of the treatment allows us to transfer one embryo, the remaining good quality embryos obtained in one IVF cycle are frozen for future use. Vitrification is thus an integral part of IVF treatment and gives our patients a greater chance of successful treatment.
Subsequent cryoembryo transfer requires only hormonal preparation of the endometrium, is painless (no need for general anaesthesia) and is also less costly.
Cryopreservation success rate
We vitrify only high quality embryos
In our clinic, we vitrify only high-quality embryos at the blastocyst stage (day 5 or 6 of culture), which guarantees up to 98% survival rate after thawing. The embryologist decides whether the remaining embryos are suitable for freezing after a personal inspection of their quality.
We were the first assisted reproduction clinic in the Czech Republic to freeze eggs by direct immersion in liquid nitrogen, the so-called open freezing system. The method is safe, the results of our one-year study showed up to 50% higher reliability and better quality of eggs after thawing than in the conventional method of freezing in a closed system, when the eggs are hidden in a carrier and frozen with nitrogen vapours.
When is it advisable to have your eggs frozen?
Freezing of sperm
Sperm freezing is a relatively simpler process compared to the above and consists of mixing ejaculate or processed sperm with a freezing medium in a certain ratio and then placing it in liquid nitrogen vapour.
Other Treatment Options
IVF with your own eggs
Many couples struggle with infertility. We know how to help.
IVF with donated eggs
We have specialized in treatment with donated eggs since the clinic was founded. Thanks to our extensive database of donors, we can choose the most suitable one for you.
IVF with donated embryos
Thanks to our sophisticated egg donation program, we have the unique opportunity to offer infertile couples treatment with donated embryos.
The first step to parenting from the comfort of your home
Our doctors are also here for you online. We provide free consultations, we will make an appointment without waiting and without the need to present a request form.