Treatment with your own eggs (IVF)
IN VITRO FERTILIZATION (IVF) involves a set of treatments in which oocytes are fertilized with sperm from a partner or a donor outside the woman's body - In Vitro Fertilization. This treatment is one of the most common methods of artificial insemination.
Who IVF treatment is for
- Couples who have been trying to conceive for several years and the cause of infertility is not found.
- Women with a diagnosed fallopian tube obstruction.
- Women diagnosed with endometriosis.
- Women who have problems with oocyte release (ovulation) and have not been successful with antiestrogens.
- Couples with a severe male sterility factor.
- Couples with an immunological factor of infertility.
By phone or online
First visit or online consultation
Stimulation protocol and medications
12 - 14 days
Oocyte retrieval + fertilization
Cultivation of embryos
5 - 6 days
Normal daily routine
Positive pregnancy test
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The IVF process
The IVF process begins with hormonal stimulation of the woman, which is necessary to obtain a larger number of oocytes than in a regular ovulation cycle. The progress of the stimulation is monitored by a doctor using ultrasound. Mature oocytes are retrieved by puncturing the follicles under general anesthesia using ultrasound control. On the day of oocyte retrieval, the partner's semen is also collected. The retrieved oocytes are then fertilised with sperm and the resulting embryos are further stored (cultured) outside the woman's body. After a few days of cultivation, the best embryo is transferred into the woman's uterus - embryo transfer. The remaining good quality embryos can be frozen and stored for further treatment.
In the first IVF cycle, 40-50% of women will become pregnant, depending on their age. With age over 40, the success rate of this method decreases.
Only what is required to get pregnant
Based on the cause of infertility, the doctor will determine the optimal course of treatment. For most couples, very simple treatment methods can help. We use the best techniques and always progress from the least difficult treatment to standard treatment protocols.
IVF with minimal stimulation
This method produces fewer oocytes than conventional IVF. In this type of stimulation, antiestrogens are used or injected at a very low dose, so this treatment is more gentle for the woman.
In practice, minimal stimulation is most often used when gonadotropin stimulation has repeatedly failed in an IVF programme or when previous stimulation has been complicated by ovarian hyperstimulation syndrome.
IVF in the native cycle
IVF without hormonal stimulation of the woman, where the goal is to obtain at least one spontaneously formed embryo. We only observe the natural growth of the follicle (oocyte) on the ovary. If it is large enough, we perform a puncture - oocyte retrieval. The oocyte is fertilized with the sperm of the partner/donor and the resulting embryo is introduced into the uterus after several days of culture.
This method has a relatively low success rate precisely because only one oocyte is retrieved. On the other hand, it is suitable for older women with a low oocyte supply or for women who do not want hormonal stimulation.
Long protocol stimulation with GnRH analogues
The long protocol used to be the gold standard of stimulation in the artificial insemination program using a combination of two types of drugs. At the beginning of stimulation, a drug used to suppress ovarian function is applied and then a drug that stimulates the ovaries is added. This method has a very good success rate with a sufficient number of oocytes being retrieved. However, it is more often complicated by the development of ovarian hyperstimulation syndrome. The success rate of the treatment is around 40% of pregnancies per embryo transfer. The protocol is suitable for young women with a good oocyte reserve. It is not suitable for women with polycystic ovary syndrome (PCOS) or for older women.
Stimulation protocol with GnRH antagonists
This stimulation protocol is currently the most commonly used stimulation protocol, has a lower consumption of stimulation hormones and the treatment takes less time than the long protocol. It also uses a combination of two types of drugs. Oocyte retrieval tends to be sufficient. The success rate of treatment is 40% of pregnancies per embryo transfer. It is particularly suitable for women with PCOS, older women and those where stimulation with the long protocol has not been successful.
How does ovarian stimulation work?
For the success of the artificial insemination treatment, it is necessary to obtain quality oocytes. The more quality oocytes we have, the higher the probability of obtaining quality embryos for embryo transfer.
The number of oocytes released is mainly influenced by the age of the patient, the state of their ovaries (ovarian reserve) and the type of stimulation protocol. Proper and controlled stimulation leads to the growth and maturation of an adequate number of follicles and the gain of a sufficient number of oocytes. It is therefore imperative to take all medications according to the treatment plan.
How are stimulant medications used?
The procedure is individual for each woman. On the 6th or 7th day of stimulation, an ultrasound examination is performed to check the number and size of individual follicles. On the basis of this examination, the stimulation dose of hormones is then increased or decreased, if necessary. Subsequently, 2 days (or 34-36 hours) before the planned egg retrieval, the woman receives an injection of hCG (Pregnyl), which is used to ripen the eggs.
How is the oocyte collection done?
Oocyte retrieval takes place 34-36 hours after the injection of hCG under ultrasound control under general anaesthesia. Each oocyte obtained is placed in a special culture dish and placed in an incubator after examination under a microscope.
On the day of egg collection, the woman arrives in the morning at a pre-determined time. From midnight onwards it is necessary to not eat, drink or smoke.
What happens after egg retrieval?
After egg retrieval, the woman remains on bed rest for about 2 hours, during which time she is informed about the number of eggs retrieved and the quality of the sperm (the partner submits a semen sample on the day of egg retrieval). After the procedure, incapacity for work can be issued.
After discharge, an escort is required, the woman is not allowed to drive the car alone. After the procedure, she starts taking the medication prescribed by the doctor. These medications are taken until the pregnancy test. In the case of pregnancy, the medication is continued until approximately the 10th week of pregnancy, unless otherwise prescribed by the doctor. Patients who are still taking other medications continue to take these medications as recommended by their doctor.
Issue of sick leave after puncture
Ask the nurse at the clinic reception and a sick note will be issued.
Embryo quality assessment
The success of fertilization is checked by an embryologist under a microscope 16-18 hours after fertilization. The resulting embryos are called pronuclei. Their number depends on the quality of the sperm, the eggs and the method of fertilisation. Assessing the quality of embryos at the pronucleus stage is very difficult. To assess the quality of the embryos, the so-called prolonged culture is used. In special media, it allows embryos to grow to higher stages. It is possible to distinguish embryos that are developing normally from embryos that are slowing down or stopping development and thus select quality embryos for transfer. The decision of which culture day is most suitable for embryo transfer depends on the number and developmental stages of the individual embryos and is entirely in the hands of the embryologist.
What is assisted hatching?
A special laboratory method that consists in disrupting the protective layer of the embryo, the so-called zona pellucida. It is mainly used for embryos before the planned preimplantation genetic testing (PGT) or in exceptional cases on the recommendation of an embryologist. For example, if the zona pellucida is too thick or hard. In other cases it is unnecessary to perform this method. A viable embryo can cope with leaving the zona pellucida and subsequent nidation on its own.
On which day is the embryo transfer performed?
The transfer of embryos to the uterus is usually carried out on day 5-6 after egg retrieval. The decision of which day of culture is the most suitable for embryo transfer depends on the number and developmental stages of individual embryos.
How does embryo transfer work?
The transfer is painless and is performed without general anaesthesia. It is similar to a normal gynaecological examination. The doctor introduces the embryo into the uterus under the direct observation of ultrasound. The patient and her partner can see their embryo on a monitor and then watch its transfer into the uterus.
Return to normal daily life after transfer
After the transfer, the woman remains lying peacefully on the ward for 10 - 15 minutes, after which the nurse prepares the required prescriptions and accompanies her to the economic department for the conclusion of treatment.
On the day of transfer and the following two days, rest is recommended. From the third day after the transfer it is possible to return to normal daily life.
Other treatment options
IVF with your own eggs
Many couples struggle with infertility. We know how to help.
IVF with donated eggs
We have been specializing in egg donation since the foundation of the clinic. Thanks to our extensive database, toghether, we can choose the most appropriate donor.
IVF with donated embryos
Thanks to our intricately designed egg donation program, we can offer you an embryo adoption too. We have a unique offer for embryo adoption too.